María Paz de Miguel González
Investigadora Senior (Contrato Miguel Servet - I2)
Jefe de Laboratorio
Hospital Universitario La Paz
Verónica Aparicio Herranz
Ayudante de Laboratorio
Hospital Universitario La Paz
María Pilar González-Peramato Gutiérrez
Facultativo Especialista de Área en Anatomía Patológica
Hospital Universitario La Paz
Diana Martín Lorenzo
Técnico de Laboratorio
Hospital Universitario La Paz
Manuel Nistal Martín de Serrano
Catedrático Departamento de Histología y Neurociencia. Facultativo de Medicina
Universidad Autónoma de Madrid
Javier Francisco Regadera González
Catedrático. Departamento de Histología y Neurociencia. Facultad de Medicina
Universidad Autónoma de Madrid
Diego Sainz de la Maza Redondo
Investigador Predoctoral
Hospital Universitario La Paz
Strategic Objective
Cellular reprogramming is a process involving the generation of pluripotent stem cells from a more differentiated cell.
The ultimate goal of research into cell engineering is the generation of induced pluripotent stem cells from more differentiated cells that can be isolated and reused autologously in patients for application in regenerative medicine.
Cell reprogramming has been approached using various methods, including somatic cell nuclear transfer, fusion with pluripotent cells, incubation with pluripotent cell extracts,
retroviral transduction using defined oncogenes, and the use of specific pluripotent prone cells such as neural stem cells and primordial germ cells. The group aims to deepen our understanding of the process of cellular reprogramming.
In addition, the group aims to identify and use stem cells for optimal tissue regeneration, using stem cells derived from the stromal vascular fraction of the human adipose tissue.
However, there are no cellular markers that identify true stem cells, which mean that the entire fraction has to be used therapeutically. This approach has several disadvantages:the therapy is not very efficient, and contaminating cells interfere with the regeneration process. We have used this whole fraction to regenerate the corneal stroma of rabbits As expected, not all of the transplanted cells were able to differentiate properly, causing interference and moderate haze in the transplanted corneas. Identifying the subpopulation with stem cell characteristics in the fraction will obviate those problems, allowing for better efficiency in transplantation, not only in the ophthalmology field, but also in many other applications.
Research Lines
• Molecular mechanisms of cellular reprogramming in normal and neoplastic environments
• New techniques and therapies in transplants